Module 6 – Data-analysis and statistics

Follow this module to learn how to correctly analyse your data and perform statistical testing

After completing it, you should be able to:

  • Explain and decide when it is useful / appropriate to use statistics and when not
  • Explain the concepts of power calculation, p-values and corrections for multiple testing
  • Demonstrate an understanding of the need to take expert advice and use appropriate statistical methods
  • Design a detailed statistical analysis plan for their study, including (but not limited to) a power calculation, in collaboration with a statistician

Core materials

Material 1 – Sample size and power

Study this material to learn about:

  • The elements that need to be taken into consideration in order to perform a power calculation/ sample size

Type: knowledge clip
Duration: 5 minutes
Created by: Healthcare triage

Material 2 – P-values

Study this material to learn about:

  • The meaning a p value

Type: knowledge clip
Duration: 5 minutes
Created by: Healthcare triage

Material 3 – The meaning of P-values

Study this material to learn about:

  • Why most published research findings are false :S
  • The meaning of statistical power
  • The effect of statistical power on the chance of false positive and false negative results

Type: knowledge clip
Duration: 5 minutes
Created by: Healthcare triage

Material 4 – Technical replicates

Study this material to learn about:

  • What technical replicates are and their implications for your power calculation

Type: knowledge clip
Duration: 5 minutes
Created by: Stats in the Lab

Material 5 – Basics of power analysis

Study this material to learn about:

  • Identify the four numerical values that are used in a conventional power analysis
  • Explain how to find information to estimate an effect size
  • Define a minimally meaningful effect size
  • Describe how to estimate what effect sizes you are able to detect when sample size is limited

Type: knowledge clip
Duration: 5 minutes
Created by: Stats in the Lab

Material 6 – Multiple testing

Study this material to learn about:

  • What multiple testing is
  • How multiple testing influences the false positive rate
  • What to take into consideration when correcting for multiple testing in your statistical analysis

Type: knowledge clip
Duration: 5 minutes
Created by: Stats in the Lab

Material 7 – Interaction effect

Study this material to learn about:

  • What an interaction effect is
  • The importance of tailoring your power calculation to interaction effects

Type: knowledge clip
Duration: 5 minutes
Created by: Stats in the Lab

Material 8 – No significant difference

Study this material to learn about:

  • How to interpret non-significant differences

Type: knowledge clip
Duration: 5 minutes
Created by: Sketchy EMB

Material 9 – Best practices in post-experimental data analysis

Study this material to learn about:

  • The benefits of pooling data across experiments done at different times, multiple time points, or different experimental groups
  • Tools to improve replicability using independent datasets and cross-validation
  • How to analyze data in which you have multiple measures from within the same person or animal, i.e. control for multiple comparisons
  • How to avoid “significance chasing,” such as interpreting or processing the data in different ways so that it passes the statistical test of significance
  • Estimations of effect size

Type: webinar
Speakers: Dr. Marcus Munafo, Dr. Deanna Barch and Dr. Damien Fair
Duration: 75 minutes
Created by:  Society for Neuroscience, featured in the Promoting Awareness and Knowledge to Enhance Scientific Rigor in Neuroscience webinar series

Bonus materials

Bonus 1 – Statistical applications in neuroscience

Study this (more advanced) material to learn about:

  • Common applications of statistics in neuroscience, including what types of research questions statistics are best positioned to address, common modeling paradigms, and exploratory data analysis.

Type: webinar
Speakers: Dr. Robert Kass, Dr. Uri Eden and Dr. Brian Caffo
Duration: 75 minutes
Created by:  Society for Neuroscience, featured in the Promoting Awareness and Knowledge to Enhance Scientific Rigor in Neuroscience webinar series

Bonus 2 – Exploratory data analysis

Study this (more advanced) material to learn about:

  • The basics of Exploratory data analysis (EDA)
  • Key tools and its role in inference

Type: weblecture
Speaker: Dr. Brian Caffo (Johns Hopkins)
Duration: 60 minutes
Created by:  University of Virginia, featured in the The Foundations of Biomedical Data Science webinar series

Bonus 3 – False Discovery Rates clearly explained

Study this (more advanced) material to learn about:

  • The concept of False Discovery Rate
  • How the Benjamini-Hochberg method corrects for multiple-testing and FDR

Type: weblecture
Speaker: Dr. Josh Starmer
Duration: 20 minutes
Created by: StatQuest


Additional resources